I’m pretty sure all the times I smoked out of a crushed soda can in high school still counteracts any benefit, but it’s good to know that wasn’t necessarily a double whammy (pending more definitive research)
Drosophila Mild Traumatic Brain Injury (mTBI) Model. TBI methods used for this study have been previously described [18,19]. Briefly, pretreated flies were anesthetized, placed in 2 mL screw cap tubes (10 flies/tube), and allowed to recover before being placed in the Omni Bead Ruptor-24 homogenizer (Omni International, Kennesaw, GA). Adult control and treated flies were pretreated for 3 days with different compounds before exposure to 10 mild trauma bouts (mild traumatic brain injury [mTBI] 10x, 2.1 m/s) at 1 week of age [19]. Following injury, flies were returned to vials containing treated media and maintained using standard culturing conditions. The number of dead flies was counted starting 48 h following trauma and then 3 times weekly for the remainder of the study, and the data were used to establish mortality indexes (MI) and longevity profiles. The percentage of dead flies described as weekly MI of treated cohorts after mTBI (10x) exposure for 1, 2 and 3 weeks was calculated. Data were normalized for total flies/cohort. Each condition includes between 110 and 235 flies (n = 110–235).
